Update, 2.30pm: Warrnambool is coronavirus free according to Department of Health and Human Services figures.
It marks the first time Warrnambool has been clear of the virus since August 14.
DHHS figures also recorded a drop of two cases in Colac-Otway Shire overnight, bringing cases in the shire to 20.
But today's report also lists 136 total cases in Colac-Otway since the pandemic began, three more than yesterday.
Half of those active cases are linked to Bulla Dairy Foods Colac, at 10, and eight to Wydinia Kindergarten in Colac.
Bulla has had a total of 20 linked cases, and Wydinia has had 16.
There remains one active case in Bendigo, and zero in Moyne, Corangamite, Glenelg, Southern Grampians, Ballarat, Surf Coast and Golden Plains Shire.
There are three active cases in Geelong and one in Horsham.
The average number of cases diagnosed in the last 14 days for metropolitan Melbourne is 49.6 and regional Victoria is 3.5
The total number of cases from an unknown source in the last 14 days is 81 for metropolitan Melbourne and one for regional Victoria.
Metro-regional border hardens as police announce new $4957 fines for rule-breakers
Earlier, 1.30pm: Metropolitan Melbourne residents found traveling unlawfully in regional Victoria will be fined $4957 under tough new state laws.
Victoria Police Deputy Commissioner - regional operations Rick Nugent announced the new rules ahead of the September school holidays.
In addition to the new fines, police will be checking every car attempting to enter regional Victoria from metropolitan postcodes.
"With restrictions easing and school holidays there's a risk people from Melbourne will travel to regional and rural areas," Mr Nugent said.
"We will be checking for potential travellers, campers, caravaners and restaurants, bars and cafes, hairdressers, beauty salons and other outlets.
"Failure to comply with the requirements to remain in restricted areas the fine will be $4957.
"That is per person, so if mum and dad are in the car, mum and dad both get a fine. That's almost $10,000."
He said police would be checking every vehicle across the state's seven permanent borders between Melbourne and regional Victoria.
Local police will be checking bus and train depots to ensure travellers are not accessing regional areas via public transport.
"We don't want the virus to spread, we want to maintain the restrictions and so we are strengthening enforcement activities particularly at borders to regional and rural areas," Deputy Commissioner Nugent said.
"We will check all vehicles, up until now we have been enabling flow through, that will change.
"There will be delays but unfortunately we need to do all we can ensure people from metropolitan Melbourne do not travel into regional and rural areas.
"There will also be additional checkpoints at Mornington to make sure people are not traveling to attend beaches."
Police will also be checking caravan parks and campings grounds, state parks, national parks and boat ramps.
"We all have restriction fatigue but we need to limit the spread of the virus, and that's by following the direction of the CHO and staying within 5 kilometres of your home.
"We need to restrict this virus and get back on with our lives."
Regional residents will be allowed to travel through metropolitan Melbourne on their way through to other regional areas, such as from Geelong to Bairnsdale.
Metropolitan residents are still allowed to visit their intimate partners in regional Victoria, but Deputy Commissioner Nugent warned people to reconsider travel.
State coronavirus cases drop below 1000 for first time in 10 weeks
Earlier, 12pm: Victoria has less than 1000 active cases, 991, for the first time in 10 weeks.
The last time the figure was under 1000 was July 9, when cases were at 932.
There are 37 cases in regional Victoria.
As of Wednesday the regional daily case average is 3.5, and 49.6 in metropolitan Melbourne.
There is one case with an unknown source in regional Victoria and 81 in metropolitan areas.
The Department of Health and Human Services has recorded 42 new coronavirus cases in the past 24 hours.
It brings Victoria's total number of cases to 19,953. Yesterday, the state had 1040 active cases including 43 in regional Victoria.
There have also been eight COVID-19 deaths overnight, all linked to aged care, bringing the overall tally to 737.
They include four women and two men in their 80s, and two men in their 90s.
There are 107 Victorians in hospital, with 11 in ICU and six on ventilators.
More than 2.5 million tests have been processed across the state, an increase of 14,374 tests since yesterday.
There are 4278 cases with an unknown source in Victoria, a decrease of four since yesterday.
Of the state's 991 active cases, 152 are healthcare workers, 37 are in regional Victoria.
Premier Daniel Andrews praised Victorians for getting cases below 1000 for "the first time in a long time".
The new cases come as regional Victoria prepares to re-open from 11.59pm tonight.
Regional Victoria's rolling 14-day average number of new cases now stands at 3.5. The last mystery COVID-19 case in regional Victoria was identified on September 1.
Victorian Premier Daniel Andrews announced regional Victoria would take the next step in the state's recovery roadmap.
Elective surgery will be scaled up across the state.
Category two and three surgeries will increase to 75 per cent in regional Victoria from tomorrow, then 85 per cent on September 28.
Elective surgeries in metropolitan Melbourne will increase to 75 per cent on September 28, and increase depending on case numbers.
All Victorian hospitals will move to 100 per cent of usual activity when the state moves to the last step to COVID Normal, planned for 23 November.
It will mean around 18,750 additional elective surgeries across private and public hospitals in October, and another 10,000 in November.
Health Minister Jenny Mikakos said hospitals would continue asymptomatic testing of healthcare staff and pre-operative COVID-19 tests.
Dental and allied health services will also be resuming.
During the third step, most dental and allied health services will be able to recommence with a COVIDSafe plan, with some limitations still in place on group therapy for allied health.
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