Charges have been laid against Spirit of Tasmania operator TT-Line and a horse transport company over the deaths of 16 polo horses in January last year.
The charges were brought against the two parties after an investigation undertaken by the Department of Primary Industries, Parks, Water and Environment.
The investigation was in relation to all aspects of the transport of the horses from Barnbougle to Victoria.
However, the horses were found dead on their arrival in Victoria after travelling on the Spirit of Tasmania.
A subsequent investigation was launched to determine the cause of the horses' deaths.
A DPIPWE spokesman said additional charges relating to unaffected horses transported during the same period that were not additionally stalled have also been laid.
"Although charges have been laid following investigation of the matter, they relate to a specific set of circumstances and the department reiterates previous advice that there is no ongoing risk for the continued movement of horses across Bass Strait in line with regulatory standards," the spokesman said.
Polo professional Andrew Williams said at the time his career and livelihood has been put on hold after the 16 horses under his management, died in transit from Tasmania to NSW.
It is understood 10 of the horses belonged to Mr Williams of Willo Polo Club and the rest were owned by his employer, Johnny Kahlbetzer, the son of German-born agribusiness baron John Dieter Kahlbetzer.
Along with his younger brother, Mr Kahlbetzer runs the family's extensive agribusiness, property, venture capital and resources operations and owns Jemalong Polo Club in NSW where the horses were based.