Senior Sergeant Tania Barbary, of Warrnambool police, said there had been 106 penalties issued across Warrnambool, Moyne, Corangamite, Glenelg and Southern Grampians shires as part of Operation Regal.

She said 50 people had been issued with a $322 fine and three demerit points for exceeding the speed limit by between 10 and 15 kilometres.

"Obviously it's a concern because speed is one of the five causations of most serious injury and fatal collisions," Senior Sergeant Barbary said.

"To have 50 of the 106 related to speed - and I consider that to be a high speed level - that's a great concern."

Senior Sergeant Barbary said police were concerned about the high number of motorists who would be using the road on Monday.

She pleaded for them to take care and have a rest if they were suffering from fatigue.

"I would urge people to take their time," Senior Sergeant Barbary said.

"If they've been celebrating the long weekend they might be fatigued and there will be a lot more users on the road.

"Take your time, don't speed or use mobile phones and pull over and have a rest if you're tired."

Senior Sergeant Barbary said four people were caught drug driving in Glenelg Shire and three people were caught drink driving - two for exceeding a zero alcohol limit and one for exceeding .05.

However, she said this was not a bad result, given that police across the region had conducted more than 1500 preliminary breath tests over the weekend.

Only one person has been nabbed using a mobile phone while driving.

Police will continue to focus on the five key causes of road trauma, being fatigue, seat belt compliance, impaired driving, speed and distraction until midnight on Monday.

The four-day road operation is also highlighting the importance of slowing down to 40km/h when passing emergency services vehicles.

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