Senior Constable Ian Hatfield, of the Warrnambool police Highway Patrol Unit, said riders from as far away as Western Australia were expected to ride through the south-west and along the Great Ocean Road as part of the Grand Prix experience.

"We would like to urge all riders to be mindful of fatigue, to plan their travel to incorporate frequent breaks and to remind riders who may be caught up in the GP to resist emerging from the track with a 'boy-racer' mentality," he said.

"There will be a strong police presence on south-west roads who will be concentration on drink driving, speed and dangerous/reckless behaviour. Heavy consequences apply to such behaviour including criminal charges, loss of licence and immediate vehicle impoundment," he said.

Victoria police across the state will be focus on speed, drink driving, vulnerable road users and public order.

With large crowds expected to be travelling to and from the island, police are reminding those attending to be responsible behind the wheel and to respect others in public.

Random breath testing sites and road blocks will be set up during operation Moto Safe, with police also using automated number plate recognition technology to detect any unregistered and unroadworthy vehicles.

Eastern region Road Policing Inspector Stewart Westfield said speed and dangerous behaviour would be targeted.

"We will have a highly visible presence and we want to put all drivers and riders on notice that we will be enforcing all speed limits," he said.

"Riders need to ensure their bike is in good, roadworthy condition and they are wearing full safety gear including helmet, gloves and quality protective clothing. We want all road users to be aware of other motorists and get to their destination safely."

Police will also have a heavy presence at the track to make the event as enjoyable as possible for attendees, and will ramp up patrols in and around Phillip Island focusing on anti-social behaviour.

Eastern region Superintendent Jock Menzel encouraged patrons to plan ahead to ensure their safety to and from the event.

"We want the event to be enjoyable for everyone. Fans will face ejection and on-the-spot-fines for being drunk, disorderly or behaving in an indecent or offensive manner," he said.

"The MotoGP will bring big crowds to the area which is positive for our region. But remember - don't drink if you plan to drive or ride. Leave it to the experts and leave speed at the track."